cation was conducted at pH 9.45. As anticipated, the spectra were shown to become very different when the metal ions(Al3+, Cr3+, Fe3+) were mixed to the ligand solution (solvent), ethanol: H2O= 4:1).
Purpose
Investigation of the Solvent Effect and pH Effect on
the Absorption, Emission and Excitation Spectra of 6-
[(2,3-Dihydroxy-benzylidene)-amino]-naphthalene-
2-sulfonic acid
r.t
*
UV Spectra
Writing Net Ionic Equations
Write the balanced molecular equation.
Write the ionic equation showing the strong electrolytes completely dissociated into cations and anions.
Cancel the spectator ions on both sides of the ionic equation
Check that charges and number of atoms are balanced in the net ionic equation
Write the net ionic equation for the reaction of silver nitrate with sodium ch
cationic 및 anionic detergent로 다시 나뉘며 이들은 highly denaturant로 monomeric protein으로 단백질을 분리시키는 특성이 있어 Western blot analysis 및 분자량 측정 등에 주로 이용한다. 그리고 Triton X-100과 같은 non-ionic detergent는 less protein denaturant이기 때문에 protein-protein interaction등에 주로 이용된다. 또한 CHAPS 등과
.
Gel Purification
1. UV로 확인한 Agarose gel의 DNA band를 잘라서 각각 1.5ml microcentrifuge tube에 넣어 무게를 잰다(pET-14b, FabG).
2. 빈 tube의 무게와 1.에서 잰 무게의 차를 이용하여 gel의 무게를 정한다.
3. Gel의 3배 만큼의 Gel binding Buffer를 첨가한다.(gel을 녹인다)
4. 60℃에서 10분간 incubation하고 2~3분마다 vortex